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. 2019 Mar 6;10:418. doi: 10.3389/fimmu.2019.00418

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Copyright © 2019 Zheng, Lou, Ding, Zhuo, Ding, Kong and Lu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of pET-28a- GRA24 in E. coli BL21(DE3) and pVAX1- GRA24 in HEK293 cell. (A) PCR amplified gra24 gene detected by 1.5% agarose gel. M: Markers; 1: Blank control; 2: coding region (CDS) of T. gondii. (B) Analysis of purified rGRA24 by SDS-PAGE. (C) The expression of pVAX1-GRA24 in HEK293 cells by an indirect immunofluorescence assay. (D) Western blotting detection of the expression of GRA24 in HEK293 cell lysates and STAg. Blank: non-transfected cells; pVAX1: empty pVAX1 transfected cells; pVAX1-GRA24 transfected cells; soluble antigen of T. gondii; rGRA24 acts as a positive control.