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. 2019 Feb 27;6(3):554–574. doi: 10.1002/acn3.730

Figure 2.

Figure 2

AZ59 disrupts the Aβo‐induced interaction of PrPC and mGluR5. (A) The binding of Myc‐tagged fly‐trap domain of mGluR5 to a 384‐well plate coated with PrPC(23‐230). Data are mean ± SEM, n = 3 replicates per sample. (B) Co‐immunoprecipitation of HEK cell lysates expressing PrPC and Myc‐mGluR5 pretreated with either vehicle, 6D11, AZ59, or control IgG before Aβo treatment. Membrane proteins were extracted with RIPA buffer and immunoprecipated with either α‐PrPC or α‐Myc. (C) Quantification of PrPC signal in (B) of α‐Myc immunoprecipitate. Data are mean ± SEM, n = 3 replicates per sample. (D) Quantification of Myc signal in (B) of α‐PrPC immunoprecipitate. Data are mean ± SEM, n = 3 replicates per sample.