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. 2019 Mar 6;10:441. doi: 10.3389/fmicb.2019.00441

FIGURE 3.

FIGURE 3

Diffusible signal factor (DSF) and pyocyanin (PYO) shows growth inhibitory effect against S. aurantiacum. Panel (A) shows the germ tube formation and elongation of S. aurantiacum CBS 136046 conidia after 8 h of co-incubation with 50 μg/ml of DSF or pyocyanin and in the control, DSF- and pyocyanin-free medium. Scale bar = 50 μm. Panel (B) shows the germination (left panel) and the growth rates (right panel) of S. aurantiacum CBS 136046 monitored over the indicated time course via microscopic and spectrophotometric analyses, respectively. The graphs show mean values with error bars indicating the standard deviations of three independent experiments. P-values were calculated using the unpaired t-test where p < 0.05 was considered to be significant (indicated with asterisks). Graphs illustrating the germination and growth rate of the other fungal strains tested are available in the Supplementary Figures 2S5S.