Table 2.
The list and composition of the media used in the study.
| Media | Components |
|---|---|
| LB | 1% NaCl, 1% peptone, 0.5% yeast extract (supplemented with 2% agar in case of solid media) |
| MEA | 5% malt extract, 1% glucose, 2% agar |
| SCFM (Kozlowska et al., 2013) | Basic components (autoclaved): 0.65% 0.2 M NaH2PO4; 0.625% 0.2 M Na2HPO4; 0.035% 1 M KNO3; 1% 119 mM diethylene triamine pentaacetic (Sigma-Aldrich); 1% porcine gastric mucin (Sigma-Aldrich), 0.14% herring sperm DNA (Sigma-Aldrich), 0.2% MOPS (PanReac AppliChem), 0.3% NaCl; 0.012% NH4Cl; 0.1% KCl; 2% agar, pH 6.8. |
| Additional components (filter sterilized with a syringe filter [0.22 μM, Merck]): 0.5% egg yolk emulsion (Sigma-Aldrich); 1% BSA (bovine serum albumin, VWR); 0.06% 1M MgCl2; 0.1% 3.6 mM FeSO4 × 7H2O; 0.175% 1 M CaCl2; 1–1% amino acid stock solutions I–VIII. | |
| Stock solutions of amino acids (AA) (pre-prepared, filter-sterilized and stored at 4°C until use): AA solution I: 53 mM L-phenylalanine, 212.8 mM L-lysine × HCl, 30.6 mM L-arginine × HCl. | |
| AA solution II: 120.3 mM L-glycine, 111.7 mM L-valine, 178 mM L-alanine, 1.3 mM L-tryptophan. | |
| AA solution III: 107.2 mM L-threonine, 144.6 mM L-serine, 166.1 mM L-proline. | |
| AA solution IV: 82.7 mM L-aspartate, 16 mM L-cysteine × HCl, 9.1% (v/v) 36.5% HCl. | |
| AA solution V: 154.9 mM L-glutamate × HCl, 80.2 mM L-tyrosine, 4% NaOH. | |
| AA solution VI: 160.9 mM L-leucine. | |
| AA solution VII: 67.6 mM L-ornithine × HCl, 63.3 mM L-methionine, 51.9 mM L-histidine × HCl. | |
| AA solution VIII: 112 mM L-isoleucine. | |
| RPMI-1640 | RPMI-1640 medium (Sigma-Aldrich) buffered with 0.165 M MOPS and supplemented with 0.03% L-glutamine |
| RPMI-1640 agar | RPMI-1640 supplemented 2% agar |
| MM | 2.5% agar; 0.05% MgSO4; 0.05% KCl; 0.05% K2HPO4; 0.001% ZnSO4; 0.001% FeSO4; 0.0003% CuSO4, 0.3% NaNO3, 3% sucrose. |
| Carbon-depleted MM | MM without sucrose. |
| Nitrogen-depleted MM | MM without NaNO3. |
| Sulfur-depleted MM | 2.5% agar; 0.05% MgCl2; 0.05% KCl; 0.05% K2HPO4; 0.001% ZnCl2, 0.001% FeCl3, 0.0003% CuCl2; 0.3% NaNO3; 3% sucrose. |
| Carbon- and nitrogen-depleted MM | MM without sucrose and NaNO3. |
| Carbon- and sulfur-depleted MM | Sulfur-depleted MM without sucrose. |
| Nitrogen- and sulfur-depleted MM | Sulfur-depleted MM without NaNO3. |
| Carbon-, nitrogen- and sulfur-depleted MM | Sulfur-depleted MM without sucrose and NaNO3. |
LB, lysogeny broth; MEA, malt extract agar; MM, minimal medium; RPMI, Roswell Park Memorial Institute; SCFM, synthetic cystic fibrosis medium.