Table 3.
Strains and plasmids used in this work.
| Strain or plasmid | Description | Reference |
|---|---|---|
| Strains | ||
| Escherichia coli | ||
| DH5α | electrocompetent cloning strain | Invitrogen |
| S17-1 | conjugating donor strain | 56 |
| Methylobacterium extorquens | ||
| AM1 | wild type; rifamycin-resistant derivative | 57 |
| exaF | ΔexaF | this work |
| fae | Δfae | 29 |
| fae exaF | Δfae ΔexaF | this work |
| fae MDH-3 | Δfae ΔmxaF ΔxoxF1 ΔxoxF2 | this work |
| MDH-3 | ΔmxaF ΔxoxF1 ΔxoxF2 | 16 |
| ADH-4 | ΔmxaF ΔxoxF1 ΔxoxF2 ΔexaF | this work |
| Plasmids | ||
| pCM157 | cre expression plasmid, TcR | 58 |
| pRK2013 | helper plasmid, IncP tra functions, KmR | 59 |
| pLB01 | Kmr, Pxox1-xoxF1-hexahistidine tag, Xa site | 5 |
| pNG284 | pLB01 with TEV cleavage site | this work |
| pHV24 | pCM184:exaF; donor for exaF::Km | 5 |
| pHV2 | SacB-based allelic exchange fae donor; KmR | E. Skovran |