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. 2019 Mar 12;9:4218. doi: 10.1038/s41598-019-40590-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Hemin-agarose pull-down assay. Cell lysates of E. coli Bl21 cells expressing LsHSCARB (residues 31–523) were mixed with hemin-agarose (Sigma-Aldrich), and samples analysed by western blot using an anti-LsHSCARB antibody (Genscript). LsHSCARB was detected in the supernatant (SN), the elution (ELU) of hemin-agarose, and the flow through (FT) of the negative control. Exposure time = 29.5 sec. The experiment was conducted three independent times with similar results.