Figure 2.

Effect of co-culture of macrophages pre-treated with CTX and the supernatant from these cells on EC migration evaluated in time-lapse assay. EC was incubated in the presence of macrophages treated or not with CTX (12.5 nM) or with supernatants from these macrophages. Then, the plate was coupled to the equipment InCell Analyzer 2200 GE in the 10x objective, for 24 h. In (A), the results are expressed as the number of migrated cells in the empty field. *p < 0.05, significantly different from mean values for group incubated with t.End. 1 (control) and **p < 0.05, significantly different from mean values for groups to their respective controls. In (B), the spacing was calculated from T0 (initial incubation up to 24 h). *p < 0.05 compared to T0, the respective groups. **p < 0.05 significantly different from mean values for culture medium and ^#p < 0.05 significantly different from mean values for Untreated MØ group. These parameters were assessed using ImageJ software Data are the average of five samples of each group ± S.E.M and represent two distinct trials. (C) is representative of response-time images obtained in real time from T0 (start of culture) and after 12 h and 24 h of the EC incubation only in culture medium (Medium), supernatant from Untreated macrophages (Control) or supernatants from CTX-treated macrophage, showing the spacing at T0 (initial time) and T24 (end time analysis).