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. 2019 Mar 12;9:4253. doi: 10.1038/s41598-019-40396-x

Figure 2.

Figure 2

The responsiveness of worker honey bees to antennal (A; n = 89) and proboscis (B; n = 23/treatment group) stimulation with water, sucrose, and sucrose contaminated with copper chloride and the effect of pretreatment with copper chloride on the sucrose response threshold in treated and control bees (C; 0 mg/L: n = 18, 0.002 mg/L: n = 19, 0.02 mg/L: n = 21, 0.2 mg/L: n = 20, 2 mg/L: n = 21, 20 mg/L: n = 17). For the assay testing the bees’ responsiveness to antennal stimulation with copper-contaminated sucrose solutions and the assay testing the sucrose response threshold following copper pretreatment, the percentages of bees exhibiting the proboscis extension reflex (% PER) are shown. For the assay testing the bees’ responsiveness to proboscis stimulation with copper-contaminated sucrose solutions, the percentages bees that consumed the whole droplet of each test solution (% consuming whole droplet) are shown. For the assay testing sucrose response thresholds in pretreated and control bees, mean DC indicates the mean value of the discrimination code. Bonferroni-corrected pairwise comparisons are indicated: ***p < 0.001, **p < 0.01, *p < 0.05.