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. 2019 Mar 12;9:4177. doi: 10.1038/s41598-019-40519-4

Figure 6.

Figure 6

Cell-cell interactions between 3DH Patient derived stromal cell lines and lung adenocarcinoma promote cancer cell proliferation and colony formation. Stromal cells were grown to a confluent monolayer for four days, and then equal amounts of red nuclear labeled A549 cells were plated atop monolayers. (A) (Upper) Phase contrast and fluorescent images of A549 grown on stromal monolayers of IMR90 and patient stroma 959. Images obtained 1 and 7 days after cancer cell seeding. (Lower) Comparison of A549 colony formation on IMR90 or 955 monolayers on day 7. (B) Comparison of A549 colony formation on hMSC, 956, and 960 monolayers. (C) Quantification of red nuclei on day 7 (mean nuclear counts were obtained from multiple images of technical duplicate plates from 2 different experiments.) Statistical analysis performed by ANOVA with Tukey’s test for multiple comparisons to the indicated groups (a: vs. IMR90, P < 0.01; b: vs WI38, P < 0.05; c: vs. hMSC, P < 0.05, d: vs. 959, P < 0.01); data presented are mean ± S.E.M. All scale bars are 100 μm.