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. 2019 Mar 12;9:4203. doi: 10.1038/s41598-019-40863-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Concentration-response curves for ponasterone A (PonA) and tebufenozide treatments against EcRs in Adoxophyes honmai. Radioactivity obtained for the treatments with excess of PonA and carrier were set at 0% and 100%, respectively. From these response curves, concentrations that inhibit the binding of [³H]PonA to 50% (IC₅₀, M) were determined by probit analysis for PonA and tebufenozide against EcRs of both wild and resistant strains. The binding activity of AhEcRB1 in tebufenozide-susceptible (A,C) or tebufenozide–resistant (B,D) strains to [³H]PonA was examined under exposure to cold PonA (A,B) or tebufenozide (C,D). Values are mean ± standard deviation (n = 3).