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. 2019 Mar 12;9:4296. doi: 10.1038/s41598-019-40639-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ENaC activity in isolated cortical collecting duct (CCD) tubules. (a) Representative current traces from cell-attached patches containing ENaC recorded from the apical membrane of split-open CCD cells of fasted or postprandial Sprague-Dawley rats. Event probability histograms of the representative traces showing the distribution of open and closed states are depicted to the right of the trace. (b) Summary graphs of ENaC activity (NP_o), channel open probability (P_o) and the average number of channels per patch from isolated CCDs. *p < 0.05, **p < 0.01, respectively, N ≥ 5 rats.