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. 2019 Mar 12;9:4165. doi: 10.1038/s41598-019-40729-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Heatmap generated after elimination of non-significant genes by ANOVA2 with Bonferroni multiple testing revealing 3 temporal patterns of differential gene expression between control and infection groups at embryonic day 18 (E18), postnatal day 2 (P2) and postnatal day 7 (P7). Pattern 1 gene expression was higher on E18 than P2 and P7 in both control and infected groups. Expression of pattern 2 genes increased from E18 to P2 in both control and infected groups and highly expressed at P7 in the infected group but not the control group. Expression of genes in patterns 3 progressively increased from E18 to P2 to P7 irrespective of the infection status of the dams.