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. 2019 Mar 12;9:4273. doi: 10.1038/s41598-019-40967-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Central distributions of synaptophysin and synaptoporin in the T7 and T13 spinal cord dorsal horn in rats. Immunohistochemistry for presynaptic vesicle proteins, synaptophysin (green) and synaptoporin (red), shows that synaptophysin is distributed across dorsal horn laminae whereas synaptoporin is localized in superficial laminae I-II. Double-labeling of synaptic terminals positive for both synaptophysin and synaptoporin is visualized as yellow in merged images. Shown are collages of 16 confocal images at 40X objective lens.