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. 2019 Mar 12;9:4273. doi: 10.1038/s41598-019-40967-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Co-localization of synaptophysin and synaptoporin with IB4-binding C fibers in the thoracic dorsal horn. IB4 binding C fibers distributed in lamina II of T7 and T13 dorsal horns and expressed synaptophysin and/or synaptoporin as shown in a representative montage of confocal images from T7. (A) Areas of IB4 binding projections within the investigated region of interest were analyzed at both thoracic levels.(B) Colocalization of IB4 binding axon terminals with synaptophysin or with synaptoporin was assessed by double labeling quantification (C) to find significantly different expression levels of synaptoporin and synaptophysin in this C fiber subpopulation (n = 4, p < 0.05, Kruskal-Wallis test with multiple comparison test, Cohen’s d > 1.3).