FIG 1.

Increased levels of IBV NA-specific plasma antibodies and plasmablasts after IIV immunization. Peripheral blood was collected at baseline and at day 7 (D7) after immunization with IIV. (A) Plasma was serially diluted, IgG specific for NA, HA, and RSV F proteins was detected by ELISA, and area under the curve (AUC) data (n = 17 subjects) are presented. (B) IBV NA and IIV (Fluzone) IgG-specific ELISpot assays were performed on total PBMC (n = 11 subjects). Representative ELISpot assay from a single subject displayed (top). Symbols represent individual subjects. Significance determined by paired t test.