FIG 5.

Clonal persistence of IBV NA hMAbs in bone marrow. Phylogenic analysis of 1092E10 (A) and 1092D4 and 1122C7 (B) IBV NA hMAb lineages was based on amino acid sequence. Lineage members are defined as exhibiting the same heavy chain V and J gene usage and HCDR3 lengths and ≥85% HCDR3 similarity. The germline sequence is represented by green diamonds, the MAb sequence is represented by blue squares, sequences obtained by MiSeq-based deep sequencing of bulk total peripheral blood B cells (D7) are represented by teal circles, bulk total bone marrow (BM) B cells (Y1) are represented by orange circles, CD138⁺ bone marrow plasma cells (Y1) are represented by red circles, sequences present in both bulk peripheral blood and bone marrow B cells are represented as purple circles, and inferred intermediate sequences are represented by gray circles. Sizes of symbols are proportional to the number of identical sequences obtained from an individual lineage member (n = 1 to 120), with the exception of the germline and hMAb sequences.