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. 2019 Mar 12;10(2):e01991-18. doi: 10.1128/mBio.01991-18

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Copyright © 2019 Edwards et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 3 — RstA represses expression of flgB reporter fusions. (A) A schematic of the flgB promoter regions for C. difficile 630 and R20291 strains. The transcriptional start site for the σ^A-dependent promoter for strain 630 lies −496 bp upstream from the flgB translational start, while the R20291 strain initiates transcription −498 bp upstream (39, 56). (B) Alkaline phosphatase (AP) activity of the promoterless::phoZ vector in 630Δerm (MC1106) and PflgB_630Δ_erm::phoZ (MC1294/MC1295) and PflgB_R20291::phoZ (MC1296/MC1297) reporter fusions in 630Δerm and the rstA::erm mutant (MC391) grown in TY medium (pH 7.4) at T₃ (three hours after the start of transition phase [OD₆₀₀ of 1.0]). The means and standard errors of the means for three biological replicates are shown. *, P < 0.05, using Student’s t test compared to the activity observed in the 630Δerm parent strain for each promoter construct.