A transposon mutant library was constructed in K. pneumoniae MH258. Distribution of transposon mutants in the MH258 genome. Track 1 (light gray, outermost) shows all coding sequences (CDSs) in the chromosomal DNA. Track 2 (dark gray) shows the CDSs in which at least one mutant was found in the library. Track 3 (black peaks, middle) shows read counts for each insertion site. Track 4 (yellow-green/purple, innermost) shows a GC skew plot using a sliding window size of 10 kb (yellow-green, above average; purple, below average). The plot was generated using DNAPlotter (49).