FIG 2.

The microbiota of the mice in the CR group were much more stable than the microbiota of the mice in the ad libitum-fed group treated with CTX. (A and B) Variation of gut microbiota structure of ad libitum group and CR group after CTX injection along PC1 and PC2 of PCoA based on the Bray-Curtis distance. (C) Clustering of gut microbiota based on distances between different groups calculated by multivariate analysis of variance test of the first 35 PCs of Bray-Curtis PCoA data. (D) Bray-Curtis distance between CTX treatment and the NS control and one-way ANOVA test were used. *, P < 0.05; **, P < 0.01; ***, P < 0.001.