FIG 3.

RDA-derived key phylotypes of the ad libitum-fed group responding to CR and CTX effects. The heat map colors of the spots in the panel represent the mean relative abundance (log-transformed) values of the OTUs in each group. The values on the color bar are the relative abundance indicated by the corresponding color. OTUs are arrayed by the Spearman correlation cluster. The key comparisons and direction of changes are summarized in three columns to the right of the heat map. A two-sided Mann-Whitney test was used for analysis. The FDR of <0.05 are shown with blue and pink box. The “ad libitum Vs. CR” column compares CR+NS mice to ad libitum+NS mice at 28d. A blue box indicates that the OTU was significantly lower in CR+NS mice, and a pink box indicates that it was significantly higher. The “NS Vs. CTX in ad libitum” column compares ad libitum+CTX mice to the ad libitum+NS mice. A blue box indicates that the OTU was significantly decreased by CTX, and a pink box indicates that the OTU was significantly increased by CTX. The “NS Vs. CTX in CR” column compares CR+CTX mice to the CR+NS mice.