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. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: Biomaterials. 2018 Aug 30;183:280–294. doi: 10.1016/j.biomaterials.2018.08.053

Figure 5. (A) Molecular mechanism of cell death and resurrection of apoptosis:

Figure 5.

Western blot analysis clearly indicates that C4.16+Sor combination completely wiped out P-AKT level both in WT and Evr-res A498 cells. Cell were either kept untreated (control, C) or treated with Evr, Sor, C4.16 or C4.16+Sor. (B) Up-regulation of caspase 7/9 with (C4.16+Sor) treatment in Evr-res A498 cells indicates effective induction of apoptosis to drug resistant cells as compared to control or individual treatment. The results support the notion that (C4.16+Sor) combination is more effective in resurrecting apoptosis mediated cell death. Data represent mean ± SD, n=3 per group, ****p<0.01 vs. control. (C) Apoptosis analysis of WT and Evr-res A498 cell by FACS using dual Annexin-V and 7-AAD staining. The data indicates CA IX-C4.16+Sor is superior in inducing apoptosis as compared to control; CA IX-C4.16 NP treated cells. (D) Histogram columns of both viable cells and apoptotic cells indicates that CA IX-C4.16 + Sor has more % apoptotic cell compared to CA IX-C4.16 alone which support our hypothesis of the synergism in RCC cell killing.