Figure 7.

Rv3463 increases the intracellular Ca²⁺ in Mycobacterium tuberculosis (Mtb)-infected macrophages. (A,B) Bone marrow-derived macrophages (BMDMs) were infected with Mtb at a multiplicity of infection (MOI) 1 for 2 h, washed three times, and incubated with or without 5 μg/ml Rv3463 for the time indicated. The cells loaded with Flou-4/AM were analyzed by confocal microscopy (A) and by flow cytometry (B). Scale bar, 10 µm. (C,D) BMDMs were preincubated for 30 min with or without 20 μg/ml Mtb LAM or a mixture of LAM and Rv3463. Ratio (340/380 nm) kinetics at different time points after the addition of 500 nM ionomycin or 5 μg/ml Rv3463. The cells loaded with Fura-2/AM were analyzed by Nikon Eclipse microscopy. Mean ± SD, n = 30 cells. *p < 0.05, **p < 0.01 and ***p < 0.001 for treatment compared to infection only. n.s., no significant difference.