FIGURE 5.

Identification of promoter regions driving expression of mpsA and mpsB. The fragments cloned into the complementing plasmids are shown below the schematic map of mob_SGI1 region. Putative promoter elements predicted by BPROM or found by manual search are indicated by blue or yellow arrowheads, respectively. Other symbols are as in Figure 1B. Transfer frequency of SGI1-C^ΔS020 and SGI1-C^ΔS019 was measured in the presence of one of the complementing plasmids and the helper plasmid R55^ΔTn6187. Plasmid pMSZ984 showed high st. deviations in three independent complementation assays carried out with 3–6 replicates with SGI1-C^ΔS019, thus the complementation efficiency of this plasmid could not exactly be determined. However, mean values appeared similar to that of pMSZ981 and pMSZ996. ^∗Transconjugants resulting from oriT_SGI1-independent transfer pathway.