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. 2018 Feb 2;10(2):141. doi: 10.3390/polym10020141

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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FCS: (A) SPAAC of aDEC205_AF647-DBCO and P(Lys)-b-P(HPMA)-N₃(stat): blue line: AF647 NHS: R_h = 0.76 nm; green line: aDEC205_AF647-DBCO: R_h = 6.0 nm; grey line: conjugate of aDEC205_AF647-DBCO and P(Lys)-b-P(HPMA)-N₃(stat): R_h = 10.0 nm (78%). (B) SPAAC of aDEC205-DBCO and P(Lys)-b-P(HPMA)-N₃(stat)_OG488: yellow line: Oregon Green 488: R_h = 0.58 nm; purple line: P(Lys)-b-P(HPMA)-N₃(stat)_OG488: R_h = 4.3 nm; grey line: conjugate of aDEC205-DBCO and P(Lys)-b-P(HPMA)-N₃(stat)_OG488: R_h = 10.7 nm (40%).