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FCS: SPAAC of polyplex by P(Lys)-b-P(HPMA)-N3(stat) and AF647 DBCO: (A) SPAAC via freeze-thaw cycles; blue line: AF647 DBCO: Rh = 0.75 nm; light grey line: SPAAC of polyplex: P(Lys)-b-P(HPMA)-N3(stat) and AF647-DBCO: Rh = 52 nm (97%). (B) SPAAC at 37 °C; blue line: AF647 DBCO: Rh = 0.75 nm; dark grey line: SPAAC of polyplex: P(Lys)-b-P(HPMA)-N3(stat) and AF647-DBCO: Rh = 50 nm (90%).
