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Fluorescence microscopy images of circulating tumor-cell isolation from blood sample of breast cancer patient on Tf-PEG substrate at 400× magnification under (a) DAPI and (b) green fluorescence filter due to anti cytokeratin (CK 18); (c) multichannel composite merged image from clinical sample (a,b); (d) bright-field merged image of captured cancer cell and (e) overlay of (c,d).
