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. 2018 Jan 1;10(1):28. doi: 10.3390/polym10010028

Figure 4.

Figure 4

Comparison of colony formation of E. coli transformed with plasmid containing text information. (A) Pictures of LB plates with ampicillin after an overnight incubation to visualize transformation efficiency. The competent E. coli DH5α transformed cells that have been diluted 1:50 were spread on ampicillin plates. (B) Comparison of transformation efficiency. One ng of each plasmid DNA sample was used for transformation of E. coli. Newly produced plasmid DNA was used as a control. A p-value < 0.05 was considered statistically significant for confidence. 1: Newly produced plasmid DNA (Control); 2: 3-year old plasmid DNA stored at −20 °C; 3–6 (AAC test): Plasmid DNA stored at 23 °C, 37 °C, 45 °C and 65 °C for 20 days.