TABLE 1:
Primer information for quantitative real-time PCR
| Gene | Forward primer 5’–3’ | Reverse primer 5’–3’ |
|---|---|---|
| β-actin | TCGACACCGCAACCAGTTCGC | CATGCCGGAGCCGTTGTCGA |
| Collagen II | AAGACGCAGAGCGCTGCTGG | GGGTCTCTACCGCGCCCTCA |
| Aggrecan | CAGCCAGGCCACCCTAGAG | GGGTGTAGCGCGTGGAGAT |
| Link Protein | ACTTCTTCTGGTGCTGATT | CTGTAGGGTCTCGGTAAA |
| COMP | TTCGGAACGCACTGTGG | TGCAGGAACCAGCGGTA |
| SOX9 | ACGCCGAGCT CAGCAAGA | CACGAACGGCCGCCTCT |
| RUNX2 | CGCACCGACAGCCCCAACTT | CTTGAAGGCCACGGGCAGGG |
| IL6 | GGCTGCTCCTGGTGATGACT | CTCCTTGCTGCTTTCACACTCA |
| IL8 | CCACACCTTTCCACCCCAAA | CCTTGGGGTTTAGGCAGACC |
| CCL2 | TCGCTGCAACATGAAGGTCT | TATAGCAGCAGGCGAC T TG G |
| CXCL12 | GCTCCACGTAGAACTGCCAT | AGGGAGAGGCTG GGA AT GAT |