Fig. 2.

WP1130 induces apoptosis in T-ALL cells. a, b Jurkat or Molt-4 cells were exposed to WP1130 for different concentrations prior to staining with Annexin-V/PI. Apoptotic cells were assessed by flow cytometry. c, d Quantitative representation of the fraction of Annexin V-positive cells treated as described for a and b were reported. e, f Jurkat or Molt-4 cells were exposed to WP1130 and the expression of apoptosis-related protein caspase 3 and PARP1 were examined by western blot. Actin expression was determined to confirm equal protein loading. Similar results were obtained for each experiment was repeated three times. **P < 0.01 vs. control group