Fig. 5.

WP1130 interacts with USP24 in cells. a Predicted binding model of WP1130 in USP24 pocket. The dashed lines in red represent hydrogen bonds between WP1130 (cyan) and USP24 (wheat). b–d CETSA was performed on indicated cells as described in methods. The thermal stabilization effect of WP1130 on USP24 and vinculin at different doses and different temperatures were evaluated by western blot. e, f The intensity of the bands of c and d were quantified by Image J software. All experiments were repeated three times with the same results