Fig. 6.

WP1130 induces apoptosis via USP24-Mcl-1 axis. a Different doses of WP1130 treated on the expression of indicated proteins in Jurkat and Molt-4 cells. Cell lysates were subjected to western blotting analysis. b Mcl-1 was immunoprecipitated from Jurkat cells in the presence and absence of WP1130 or knockdown of USP24, and the indicated proteins were examined by western blot. c The indicated cells were treated with WP1130 in the presence or absence of MG132, and the protein level of USP24 and Mcl-1 were then examined by western blotting. d Jurkat or Molt-4 cells were exposed to DMSO or WP1130 for 24 h. The collapse of mitochondrial transmembrane potential was assessed by flow cytometry using RH123 staining. e–g Jurkat cells were transfected with the vector or dCas9-sgUSP24 plasmid, followed by western blot assay. Indicated cells were treated with WP1130 and the cell viability was evaluated by trypan blue exclusion assay and the indicated protein was examined by western blot. h Jurkat cells were transfected with the vector or V5-Mcl-1 plasmid and then treated with WP1130 and the indicated proteins were examined by western blot. Similar results were obtained for each experiment was repeated three times. **P < 0.01 vs. control group