Table 1. List of oligonucleotide sequences used in the study.
| Assay name | Oligonucleotide name | Oligonucleotide sequence (5’–3’) | Amount in nM | Method |
|---|---|---|---|---|
| FluC NP qPCR | FluC NP F1068 | GCRTGCTTTGGRCTTGCTTATG | 600 | qPCR |
| FluC NP R1161 | ARTTTCCTATTTTCATTCTGTTTCTCAAC | 600 | ||
| FluC NP TM1100 | FAM – TTTGGTYTCTGCYATGGTYAGCCAYCCTCT - BHQ1 | 200 | ||
| FCV qPCR (IC) | FCV F54 | CGTTACCGCCACACCCAT | 300 | qPCR |
| FCV R141 | GAGTTCACGAAAGATTTCAGACCAT | 300 | ||
| FCV TM96 | LC610 - ACCCATCATTCTAACACTCCCGCCAAT - BHQ1 | 100 | ||
| HE fragment 1 | FluC HE F1 | AGCAGAAGCAGGGGKTTAATAATG | 500 | nPCR |
| FluC HE F7 | AGCAGGGGTTTAATAATGTTTTTCTC | 500 | ||
| FluC HE R866 | CCAGAATTCCCTGTGTAAGGTGA | 500 | ||
| FluC HE R895 | ATCATGTCACATTGCATTGTTGG | 500 | ||
| FluC HE F477 | CCAGAAAARCATYTATGAATTGGC | 500 | Seq | |
| FluC HE R353 | CCAGGTGGGCCAAACATACT | 500 | ||
| HE fragment 2 | FluC HE F714 | GCATCTTGTGGCTTCTTGCTATT | 500 | nPCR |
| FluC HE F722 | TGGCTTCTTGCTATTTCATYTATGAYAG | 500 | ||
| FluC HE R1474 | CTTTTGTYACACCTCCTCCTGAT | 500 | ||
| FluC HE R1511 | TCATTTCCAATTTTYTCRAAYCC | 500 | ||
| FluC HE F1133 | ATAAAGAAATGAGGGACYTGCTGT | 500 | Seq | |
| FluC HE R1085 | ATYARCATGCACCCTGGAGTG | 500 | ||
| HE fragment 3 | FluC HE F1327 | ACTGATACCACTGTAACCAAACCYAA | 500 | nPCR |
| FluC HE F1338 | TGTAACCAAACCYAARAGCAGRAT | 500 | ||
| FluC HE R2067 | AGCAAGGGGATTTTTGTTTTTYATAA | 500 | ||
| FluC HE R2073 | AGCAGTAGCAAGGGGWTTTTTGT | 500 | ||
| FluC HE F1703 | TGTGGGAACTAGCTTCAGAAATAAC | 500 | Seq | |
| FluC HE R1663 | AGGCTCTTATTATYCCCAATTCTCC | 500 | ||
| cDNA synthesis | Uni11 | AGCAGAAGCAG | 1,000 | Seq |
BHQ1: black hole quencher 1; FAM: fluorescein amidite; FCV: feline calicivirus; HE: haemagglutinin esterase; IC: internal control; LC610: LightCycler 610; NP: nucleoprotein; nPCR: nested PCR; qPCR: quantitative/real-time PCR; seq: sequencing.