. Author manuscript; available in PMC: 2019 Mar 13.

Published in final edited form as: Neurobiol Dis. 2018 Jul 6;118:76–93. doi: 10.1016/j.nbd.2018.07.004

Table 1.

Passive properties of CHO hm1 cells transfected with GFP and K_v7.2 containing epileptic encephalopathy mutations.

	Without diC8-PIP₂				With diC8-PIP₂
Transfection	n	V_m (mV)	C_m (pF)	E_rev (mV)	n	V_m (mV)	C_m (pF)	E_rev (mV)
GFP	9	−10.2 ± 0.7*	11.9 ± 2.1	−25.6 ± 1.6*	ND	ND	ND	ND
GFP + K_v7.2 WT	11	37.0 ± 1.1	14.4 ± 1.5	−37.0 ± 1.1	8	−36.6 ± 1.5	17.2 ± 1.1	−44.6 ± 1.7‡
GFP + K_v7.2 A343D	12	−9.2 ± 0.5*	13.2 ± 0.7	−25.5 ± 2.3*	ND	ND	ND	ND
GFP + K_v7.2 R333W	12	−39.3 ± 1.4	14.7 ± 1.3	−40.5 ± 1.9	13	−37.1 ± 1.4	17.1 ± 1.4	−44.7 ± 2.1‡
GFP + K_v7.2 M518V	8	−28.5 ± 1.6*	22.0 ± 5.9	−29.9 ± 3.7*	ND	ND	ND	ND
GFP + K_v7.2 K526N	14	−37.1 ± 1.4	17.7 ± 3.0	−43.1 ± 0.8*	14	−42.7 ± 1.8†‡	17.8 ± 1.3	−53.1 ± 2.2†‡
GFP + K_v7.2 R532W	18	−35.3 ± 1.3	17.8 ± 1.9	−37.0 ± 1.9	11	−36.3 ± 1.2	17.6 ± 1.2	−45.0 ± 1.2‡

n, number; V_m, resting membrane potential; C_m, whole cell membrane capacitance; E_rev, reversal potential. Ave ± SEM (*p<0.05 compared to K_v7.2 wild-type (WT); †p<0.05 for K_v7.2 WT + diC8-PIP₂. vs mutants + diC8-PIP_2; ‡p<0.05 for K_v7.2 WT vs mutants + diC8-PIP₂). ND, not determined. Each value represents the Ave ± SEM.