Table 2. Properties of Metarhizium anisopliae GH20 and GH3 β-N-acetylglucosaminidases.
| Identification | GH family | ORF length (nt) | Introns | Protein length (aa) | Mature protein theoretical kDa | Theoretical pI | Conserved domain | Transmembrane domain | Signal peptide | GPI or NCS | N-glycosylation site | Acession number |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| MaNAG1 | GH20 | 1,863 | 0 | 620 | 66.98 | 6.07 | cl02948 / pfam14845 | - | + | - | 4 | KFG80340 |
| MaNAG2 | GH20 | 1,862 | 2 | 579 | 61.42 | 4.85 | cd06562 / pfam14845 | - | + | - | 4 | KFG85702 |
| MaNAG3 | GH3 | 2,730 | 4 | 909 | 98.71 | 6.25 | COG1472 | + | - | - | 6 | KFG78085 |
| MaNAG4 | GH3 | 1,701 | 1 | 566 | 60.67 | 5.6 | COG1472 | - | - | - | 2 | MANI_ 128875 |
(+): presence; (-) absence; pI: isoelectric point; GPI: GPI-anchor sites; NCS: non-classical secretion pathway regions.