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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: J Cell Physiol. 2018 Aug 5;234(2):1426–1441. doi: 10.1002/jcp.26948

Fig. 3:

Fig. 3:

Knockdown of IGF-1R attenuates IGFBP-3 secretion in HCECs. Secreted IGFBP-3 in conditioned media was measured using an IGFBP-3 ELISA. (A) HCECs were treated with siRNA oligonucleotides targeting IGF-1R. In basal media, IGF-1R knockdown resulted in a large decrease in secreted IGFBP-3 compared to the non-targeting control (***P<0.001, Two-way ANOVA, Holm-Sidak post hoc multiple comparison test). (B) Immunoblotting for IGF-1R confirmed knockdown in whole cell lysates. β-actin was used as a loading control. KGM: keratinocyte growth media; KBM: keratinocyte basal media; CTRL: control; ins: insulin. Data representative of 3 independent experiments performed in triplicate. All independent experiments used HCECs derived from different donors. ELISA data presented as mean ± standard deviation.