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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: J Cell Physiol. 2018 Aug 5;234(2):1426–1441. doi: 10.1002/jcp.26948

Fig. 8:

Fig. 8:

SUMO-2/3 but not SUMO-1 triggers IGF-1R nuclear translocation and DNA binding in corneal epithelial cells. (A) Immunoblotting for SUMO-1 and SUMO-2/3 showed a decrease in SUMO-1 in basal media compared with growth. Instead in basal media, there was an observable increase in SUMO-2/3. Data representative of 3 independent experiments. (B) Proximal Ligation Assay (PLA) shows an increase in IGF-1R SUMOylation by SUMO-2/3 (green) in the cytoplasm and nucleus in basal media compared with growth media. Scale bar: 23 μm. Data representative of 3 independent experiments. (C) The global SUMOylation assay confirmed a significant decrease in SUMOylated IGF-1R in nuclear extracts after knock down of IGFBP-3 in basal media (siRNA control versus siRNA IGFBP3 *P<0.05, siRNA IGFBP3 plus BP3 versus siRNA control and siRNA IGFBP3 in basal ***P<0.001, One-way ANOVA, Holm-Sidak multiple comparison test). KGM: keratinocyte growth media; KBM: keratinocyte basal media; BP3: IGFBP-3; CTRL: control; PI: propidium iodide. Data representative of 4 independent experiments performed in triplicate. Data shown as mean ± standard deviation.