Table 1.
Primers sequences and accession codes used in quantitative PCR analysis
| Gene Name | Accession | Primer Sequence (5′-3′) | Product Size, bp | Tm, °C | Efficiency |
|---|---|---|---|---|---|
| NAGS | NM_001097520.1 | F: CAGGCTGTCTTTCCTTCTGG R: CACAGGATGGGAATGCTACC |
226 | 60 | 99.0 |
| PDG* | AF490841.1 | F: AAGGCACAGACATGGTTGGT R: AAGCAAACTGCCCTGAGAAA |
213 | 60 | 97.4 |
| OTC* | NM_001164002.2 | F: GCCTTTGTGAACAAGGTGGT R: TGAGATGCATGACAAGCACA |
135 | 60 | 99.3 |
| OAT* | NM_001185141 | F: CCAGTTACGACGGGTTTGGA R: GAACGCAGCAACGTTTGGAT |
107 | 60 | 97.8 |
| ARG2* | XM_001928679.3 | F: TACGTCCTGCCCTTCGTATC R: CAAGCCAGCTTCCCTTACAG |
182 | 60 | 95.7 |
| ASS* | XM_005660523.1 | F: GCTGGTGTACACGGGTTTCT R: CCAGCTCCTCGTTGTAGAGG |
167 | 60 | |
| CPS1* | XM_005672159.1 | F: CTGAGGCCCAGACAAGGGAA R: TGCAGCCAGTGTATCAATCTGT |
77 | 61 | 102.0 |
| P5CS* | XM_005671336.1 | F: CGGAACCTCAATGGGACACT R: CCTTAACACTAATAACCCCCTGGA |
127 | 60 | 95.3 |
| POX/PRODH | AK231562.1 | F: CCGATCGCCAGGTGTACTTT R: CTCCATCACGGGGCCATAAG |
115 | 60 | 93.9 |
| NOS3* | NM_214215.1 | F: CAGCCCTGATGGAGATGTCG R: GGTCTGAGCAGGAAACGCTA |
99 | 60 | 99.5 |
| ASL* | XM_003124443.2 | F: AGTTCCTGTTCTGGGCTTCG R: GCTTCCAGTGCTGTAGGCAT |
122 | 60 | 105.3 |
| ARG1* | NM_214048.2 | F: GGCTGGTCTGCTTGAGAAAC R: AGCCAGCTGTTGATTTGCTT |
144 | 60 | 105.3 |
F, forward; NAGS, N-acetylglutamate synthase; POX, proline oxidase; PRODH, proline dehydrogenase 1; R, reverse; Tm, temperature.
*
Gene definition is provided in the manuscript.