Table 3.

Characteristics and receptor gene expression of AT excised from epididymal WAT and subscapular BAT from mice fed NC and HF diets

	WAT		BAT
	NC	HF diet	NC	HF diet
	Characteristics
Maximal diameter, µm	181 ± 5	197 ± 10	179 ± 23	163 ± 9
Wall thickness, µm	21 ± 2	19 ± 2	20 ± 1	20 ± 1
Wall-to-lumen ratio	0.12 ± 0.01	0.10 ± 0.01	0.11 ± 0.01	0.12 ± 0.01
Spontaneous tone, %	1.59 ± 1.37	1.48 ± 0.66	7.86 ± 3.46	6.47 ± 3.46
EC50, log M
ANG II	−8.09 ± 0.56	−8.55 ± 0.24	−8.19 ± 0.13	−8.29 ± 0.77
Norepinephrine	−6.65 ± 0.30	−6.24 ± 0.33	−6.02 ± 0.28	−4.98 ± 0.79
Endothelin-1	−9.67 ± 0.28	−9.05 ± 0.32	−8.78 ± 0.32	−9.38 ± 0.27
			(P = 0.06)
KCl	−3.45 ± 0.43	−3.35 ± 0.38	4.29 ± 0.29	−4.24 ± 0.25
	Receptor expression
Agtr1a	1 ± 0.26	0.85 ± 0.27	3.58 ± 1.2	0.95 ± 0.37
Adrβ3	1 ± 0.24	0.65 ± 0.16	0.36 ± 0.15*	0.63 ± 0.31
Adrα1	1 ± 0.13	1.67 ± 0.47	4.87 ± 1.26*	7.67 ± 2.35†
Adrα2	1 ± 0.18	1.05 ± 0.11	0.13 ± 0.07*	0.08 ± 0.03†
Ednra	1 ± 0.12	0.81 ± 0.12	0.09 ± 0.02*	0.11 ± 0.03
Ednrb	1 ± 0.13	0.76 ± 0.13	0.09 ± 0.02*	0.10 ± 0.03

Values are means ± SE. Receptor expression values were calculated by measurement of $2^{(-\Delta {\text{C}}_{\text{T}})}$, where C_T is threshold cycle, and normalized to mean values of the test gene in white adipose tissue (WAT) in normal chow (NC)-fed mice. BAT, brown adipose tissue; ANG II, angiotensin II; Agtr1a, ANG II receptor type 1a; Adrβ3, adrenergic receptor-β₃; Adrα1, adrenergic receptor-α₁; Adrα2, adrenergic receptor-α₂; Ednra, endothelin receptor type A; Ednrb, endothelin receptor type B.

^*P < 0.05 vs. NC-WAT;

^†P < 0.05 vs. HF-WAT.