Table 1. Behaviour monitoring timings.
Start time of monitoring of behavioural responses to stressor treatments for each experimental phase. 10 minutes of behavioural video footage was collected during each monitoring period.
| Monitoring period start time (hh:mm after start of treatment) | ||||||
|---|---|---|---|---|---|---|
| Phase | Monitoring period code1 | Monitoring period description | Control | Crowding | Hypoxia | Crowding & hypoxia |
| September 2015 | P | Pre-treatment | -01:25 | -02:36 | NA2 | -01:28 |
| T1 | Start of treatment | 00:00 | 00:00 | NA2 | 00:00 | |
| T2 | During treatment A | 01:31 | 01:06 | NA2 | 01:29 | |
| T3 | During treatment B | 02:35 | 02:01 | NA2 | 02:31 | |
| M4 | 1 day post treatment | 22:40 | 26:17 | NA2 | 28:31 | |
| M5 | 2 days post treatment | 48:18 | 44:36 | NA2 | 46:46 | |
| M6 | 3 days post treatment | 70:16 | 70:22 | NA2 | 70:27 | |
| M8 | 6 days post treatment | 142:14 | 143:35 | NA2 | 141:51 | |
| September 2016 | P | Pre-treatment | -01:00 | -01:17 | -01:00 | -01:20 |
| T1 | Start of treatment | 00:00 | 00:00 | 00:00 | 00:00 | |
| T2 | During treatment A | 01:15 | 01:05 | 01:16 | 01:15 | |
| T3 | During treatment B | 01:45 | 01:40 | 01:45 | NA3 | |
| M1 | ~0.5 hours after end of treatment | 02:40 | 02:40 | 02:44 | 02:05 | |
| M2 | ~2 hours after end of treatment | 04:10 | 04:10 | 04:05 | 03:35 | |
| M3 | ~4 hours after end of treatment | 06:30 | 06:18 | 06:05 | 05:50 | |
| M4 | 1 day post treatment | 28:40 | 29:45 | 28:50 | 26:55 | |
| M5 | 2 days post treatment | 50:18 | 48:50 | 48:30 | 46:55 | |
| M6 | 3 days post treatment | 71:20 | 73:55 | 74:30 | 70:50 | |
| M7 | 4 days post treatment | 95:55 | 96:43 | 94:05 | 95:18 | |
| M8 | 5 days post treatment | 117:59 | 118:15 | 118:00 | 117:59 | |
| October 2016 | P | Pre-treatment | -01:00 | -01:00 | -02:15 | -00:55 |
| T1 | Start of treatment | 00:00 | 00:00 | 0:00 | 00:00 | |
| T2 | During treatment A | 01:00 | 01:00 | 01:40 | 00:15 | |
| T3 | During treatment B | 02:00 | 02:00 | 02:35 | 00:31 | |
| M1 | ~0.5 hours after end of treatment | 03:52 | 02:50 | 03:25 | 01:15 | |
| M2 | ~2 hours after end of treatment | 05:27 | 04:15 | 04:55 | 02:45 | |
| M3 | ~4 hours after end of treatment | 07:02 | 06:14 | 06:55 | 04:45 | |
| M4 | 1 days post treatment | 24:45 | 27:30 | 27:50 | 23:40 | |
| M5 | 2 days post treatment | 50:00 | 48:20 | 48:22 | 51:15 | |
| M6 | 3 days post treatment | 75:50 | 74:30 | 71:35 | 75:55 | |
| M7 | 4 days post treatment | 96:10 | 99:05 | 96:25 | 98:35 | |
| M8 | 5 days post treatment | 119:30 | 121:25 | 120:40 | 120:30 | |
1Monitoring period codes consist of either; “P” for pre-treatment; “T” for during treatment or “M” for post treatment monitoring
2Hypoxia treatment was not applied during the September 2015 phase
3There was insufficient time to collect a third monitoring period (T3) during treatment before oxygen levels fell below acceptable welfare levels