Fig 10. Alternative splicing analysis of Clcn7 mRNA.

(A) RT-PCR using primers that amplify a region spanning exon 6 to exon 25 was performed from bone total RNA of wild-type (Clcn7^WT/WT) and Clcn7^Flox/Flox mice and analysed by 1.5% agarose gel. In wild-type animals one band, 1683 bp long, corresponding to the correctly spliced Clcn7 transcript including 20 coding exons is present. This band is not detected in mutant mice, conversely a 1216 bp long band lacking exon 7–11 is observed.