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. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: J Cell Physiol. 2018 Jun 1;233(11):8418–8428. doi: 10.1002/jcp.26801

FIGURE 8. TGFβ1- and TGFβ3-induced EndMT needs activation of a paracrine loop in HMECs involving TGFβ2.

FIGURE 8

(A) Representative Western blot images showing increased expression of the most potent EndMT stimulating TGFβ isoform, TGFβ2, in response to 1, 2.5 and 5 ng/ml of TGFβ1, TGFβ2, and TGFβ3 for 72 hours. (B) Bar graph of Western blot band densitometry analysis showing increased expression of TGFβ2 in response to 1, 2.5 and 5 ng/ml of TGFβ1, TGFβ2, and TGFβ3 for 72 hours. (C) Representative Western blot images showing increased expression of N-Cadherin, αSMA, and TGFβ2 by both TGFβ1 and TGFβ3, both of which were blunted upon SiRNA-mediated knockdown of TGFβ2. (D–E) Bar graph of Western blot band densitometry analysis showing increased expression of N-Cadherin and αSMA by both TGFβ1 and TGFβ3, which were blunted upon SiRNA-mediated knockdown of TGFβ2. Data are represented as mean ± SD. (n=3–5), *p<0.05; #p<0.01.