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. 2018 Dec 4;139(11):1407–1421. doi: 10.1161/CIRCULATIONAHA.118.036652

Figure 2.

Figure 2.

Propionate provides beneficial modulation of effector T cells in AngII-infused ApoE–/– mice. A, AngII-infused ApoE–/– mice were treated with C3 or sodium chloride, starting 5 days before minipump implantation. B, Survival curves of AngII-infused ApoE–/– mice treated with C3 or control. n=30 per group, ***P<0.001 by log-rank test. C, After 28 days of AngII infusion, splenocytes were analyzed for CD4+ effector memory (CD44+CD62L–) and naive (CD44–CD62L+) subsets. Left, Representative flow cytometry plots. Right, Quantification in percentage of CD4+. ApoE–/– AngII n=15, ApoE–/– AngII+C3 n=19. D, Quantification of FoxP3+CD25+ and RORγt+ in CD4+ splenocytes. Left, Representative flow cytometry plots. Right, Quantification in percentage of CD4+. ApoE–/– AngII n=12, ApoE–/– AngII+C3 n=15. *P<0.05, **P<0.01, by 1-tailed t test. AngII indicates angiotensin II; APC, Allophycocyanin; ApoE–/–, apolipoprotein E knockout–deficient; C3, propionate; FITC, fluorescein isothiocyanate; and PerCP-Cy5.5, Peridinin-chlorophyll protein cyanine 5.5.