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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: Biomed Chromatogr. 2019 Jan 16;33(4):e4461. doi: 10.1002/bmc.4461

Table 1.

HPLC gradient programa

Minutes B %
0-1 10 (isocratic)
1-4 10-90 (linear)
4-9 90 (isocratic)
9-10 10 (linear)
10-17 10 (isocratic)
a

Mobile phase A was 2% acetonitrile with 0.1% formic acid and mobile phase B was 100% acetonitrile with 0.1% formic acid