Figure 1.

Modeling tumor-lymph node interactions on a dual-slice microfluidic chip with continuous recirculating flow. (a) In vivo, tumors and lymph nodes communicate via lymphatic fluid drainage from the tumor to lymph node, and via blood flow that carries signals and cells from the lymph node back to the tumor. (b, c) A simplified conceptual schematic (b, top view; c, side view of tissue chamber) of the dual-slice chip. Arrows indicate the direction of fluid flow. The tissue sample rests on a track-etched membrane, and flow passes through it on the way to the next chamber.