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. 2019 Mar 7;10:380. doi: 10.3389/fimmu.2019.00380

Figure 5.

Figure 5

SAA promotes kidney inflammation in ApoE−/− mice. ApoE−/− mice were administered SAA, LPS or sterile PBS for 2 weeks (as described in Study 1). After a further 2 weeks mice were sacrificed and kidney sections from control, LPS- and SAA-treated mice were stained with PAS. Data represent at least 3 different fields within each kidney section obtained from n = 8 (control or LPS) or 10 (SAA) animals. Arrows highlight condensed glomeruli in renal samples from SAA-treated mice. Magnification x200, scale bar = 200 μm. The change in Bowman's space (A) expressed as a percentage of the corresponding total glomerular area was calculated as defined in the Methods. Each point represents mean data from glomeruli present in a field of view (at least 3 fields of view; FOV) obtained at 200x magnification as assessed with Image-pro Plus (V6). Data represent mean ± SD; n = 8 (Control) or 8 (LPS-) and 10 SAA-treated mice. *Different to vehicle- or LPS-treated mice in the absence of SAA; P < 0.05; ns, not significant. Renal homogenates were assayed simultaneously for 3-chlorotyrosine and total tyrosine content (B). Quantitative mass data represent mean ± SD; n = 5 (Control) or 4 (LPS- and SAA-treated) mice. *Different to vehicle- or LPS-treated mice in the absence of SAA; P < 0.05.