Figure 8.

SAA promotes TF accumulation in renal tissues. ApoE^−/− mice were administered SAA, LPS or sterile PBS for 2 weeks (as described in Study 1). Tissue sections obtained from vehicle- (control), LPS- and SAA–treated mice were co-stained with an FITC-conjugated anti-TF antibody (Upper) or with DAPI alone (corresponding Lower) to highlight the nuclear envelope then imaged with an Olympus fluorescence microscope. No TF⁺ immune-reactivity was detected in kidney tissue from control or LPS-treated mice. Arrows indicate clusters of DAPI-labeled nuclei localized to glomeruli that are detected in lower panels and reflected as a corresponding arrow in the corresponding panel above to indicate glomeruli in the same renal section. Data are representative of at least three different fields of view (×200 magnification) from each kidney section taken from n = 8 (control or LPS) or 10 (SAA) mice. Digital inset shows a representative overlay of DAPI and FITC fluorescence within a glomerulus (400× magnification); scale bar, 200 μm.