Table 3.
Kidney tissue concentrations of native and oxidized lipids and vitamin Ea.
| Controlb | LPS | SAA | Control | LPS | SAA | |
|---|---|---|---|---|---|---|
| n = 8 | n = 8 | n = 10 | n = 8 | n = 8 | n = 10 | |
| 4 weeks after starting SAA-treatment | 18 weeks after starting SAA-treatment | |||||
| [FC] nmol/mg P | 1.7 (0.3) | 1.6 (0.4) | 1.7 (0.3) | 1.9 (0.5) | 1.9 (0.5) | 1.8 (0.6) |
| [TOH] pmol/mg P | 127.8 (30.9) | 132.9 (31.7) | 125.2 (42.9) | 142.2 (60.2) | 138.2 (46.1) | 154.6 (55.8) |
| [CeO(O)H] pmol/mg P | 4.0 (2.3) | 1.9 (0.9) | 18.3 (4.7)*# | 1.4 (2.0) | 2.7 (1.4) | 24.4 (17.7)* |
| [C18:2] nmol/mg P | 0.3 (0.02) | 0.3 (0.02) | 0.3 (0.3) | 0.4 (0.2) | 0.4 (0.2) | 0.5 (0.2) |
| [C20:4] nmol/mg P | 0.9 (0.4) | 1.1 (0.6) | 1.0 (0.5) | 2.1 (2.2) | 1.2 (0.4) | 1.6 (0.7) |
| [F2-isoprostanes] pg/mg P | 21.2 (4.7) | 28.5 (9.6) | 66.9 (7.8)*, # | 35.3 (7.1) | 40.2 (7.1) | 51.8 (6.1)*# |
Mice received normal chow and were administered vehicle, LPS or SAA every 3 days over 14 days by i.p. injection (for details see Materials and Methods). At 4 or 18 weeks after SAA treatment, kidneys were harvested, and the following parameters measured in prepared renal tissue by liquid chromatography or an ELISA kit (F2-isoprostanes). Unesterified cholesterol, FC; α-tocopherol (biologically active vitamin E), α-TOH; cholesteryl linoleate, C18:2; cholesteryl arachidonate, C20:4; combined cholesteryl esters (C18:2 and C20:4), CE; CE-derived lipid hydroperoxides and hydroxides, CeO(O)H.
Units of measurement are as indicated. Data represent Means ± (SD); n–as indicated.
Different to the control; P < 0.05.
Different to the corresponding LPS group; P < 0.05.