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. 2019 Mar 7;10:169. doi: 10.3389/fphar.2019.00169

Figure 2.

Figure 2

Mass spectrometric optimization of electronic parameters as well as collision gas and source temperature in SRM mode for compounds RvE1, RvD2, and RvD5. Shown is the influence of parameters on signal intensity within a range around the optimum value. (A) Source temperature (TEM) between 300 and 550°C, (B) declustering potential (DP) between −20 and −100 V (C) collision energy (CE) in steps of 2 V from −13 to −31 V for RvE1 (m/z 349 → 195) and RvD5 (m/z 359 → 199), CE from −21 to −39 V for RvD2 (m/z 375 → 175) with collision activated dissociation (CAD) gas set to high (15 psi), (D) collision cell exit potential (CXP) in steps of 2 V from −4 to −18 V, (E) collision energy (CE) in steps of 2 V from −13 to −31 V for RvE1 (m/z 349 → 195) and RvD5 (m/z 359 → 199), CE from −21 to −39 for RvD2 (m/z 375 → 175) with collision activated dissociation (CAD) gas set to high (15 psi), medium (9 psi) and low (6 psi).