Figure 1.

Transcriptional profile of lung CD8⁺ T_RM cells arising after influenza virus infection. (A) Representative flow cytometry plots are shown of CD69 and CD103 expression on memory (CD44^high CD62L^lo) CD8⁺ T cells isolated from lungs of naïve mice or lungs of mice at day 30+ (memory phase) after intranasal HKx31 influenza virus infection. (B) Expression (column Z-score) of mRNA from genes belonging to the T_RM core signature (12) in CD69⁺ and CD69⁻ memory (CD44^high CD62L^lo) CD8⁺ T cells isolated from murine lungs at day 30+ after HKx31 infection. Genes in bold are differentially expressed in CD69⁺ vs. CD69⁻ cells. Expression of gene Usp33, part of the T_RM core signature, was not detected by our analysis. (C–G) Expression (log2 read counts per million, voom normalized expression) of (C) Hobit (Zfp683) and Blimp-1 (Prdm1), genes encoding for (D) common γ-chain cytokine receptors, (E) cytokines, (F) cytotoxic mediators, and (G) selected chemokines is shown in CD69⁺ and CD69⁻ memory (CD44^high CD62L^lo) CD8⁺ T cells isolated from murine lungs at day 30+ after HKx31 infection. Symbols represent individual mice and line indicates the mean. Data from one experiment (n = 8), taken from Hombrink et al. (23). ^*FDR adjusted P < 0.05; ^*** FDR adjusted P < 0.001; ns: not significant.