Skip to main content
. 2019 Mar 7;10:400. doi: 10.3389/fimmu.2019.00400

Figure 5.

Figure 5

CD8+ TCM formation is suppressed by Blimp-1. Mixed bone marrow chimeras [WT:WT; WT:Hobit KO (HKO); WT:Blimp-1 KO (BKO)] were infected intranasally with HKx31 influenza virus. The phenotype of virus-specific (Db NP366+) WT (CD45.1+), WT (CD45.2+), HKO (CD45.2+) and BKO (CD45.2+) donor CD8+ T cells was analyzed in blood (A–D) and in the indicated tissues (E). (A,C) Representative flow cytometry plots show expression of CD44 and CD62L on Db NP366+ donor CD8+ T cells from the blood at (A) day 10 and (C) day 30+ post infection. (B,D,E) Frequencies of CD44highCD62L+ cells Db NP366+ donor CD8+ T cells from (B) blood at day 10 post infection, and from (D) blood as well as from (E) spleen, mediastinal lymph nodes (mLN) and lung at day 30+ post infection were quantified. Combined data from two independent experiments (n = 9–10). Symbols represent individual mice, lines connect paired samples. Paired t-test. *P < 0.05; **P < 0.01; ***P < 0.001, ns: not significant.